The CD45 marker is also known as an LCA (leukocyte common antigen). It is a surface marker and also a transmembrane protein. It is expressed by tumors of a leukocytic lineage, and the leukocytes. It is designed to be used for research and shouldn’t be used for any procedures. It has a clone of SP307. The immunogen is the synthetic peptide from the human CD45 protein c-terminus. The isotype is the Rabbit IgG, and it has an undetermined epitope. Likewise, the molecular weight ranges from 180 to 240kDa.
Applications
The CD45 marker can be used in Immunohistochemistry, Western Blotting, and Flow Cytometry applications.
For IHC applications, you can use paraffin-embedded or formalin-fixed tissues. Deparaffinized slides are required, as well. You can choose the concentrated version of the product, which means you should dilute the antibody using a ratio of 1:100. You can also find a pre-diluted format. To retrieve the antigen, you should boil a tissue section in an EDTA buffer with a pH of 8.0 for at least 10 minutes. Let it cool to room temperature for 20 minutes before beginning the incubation period of 10 minutes, also at room temperature. The positive control is the tonsil.
For Western Blotting applications, you should dilute the antibody using a ratio of 1:3200 and incubate for one hour at room temperature. It is not recommended to use the pre-diluted formula for Western Blotting. The positive control is the Jurkat Cell Lysate.
For Flow Cytometry applications, you should dilute the antibody using a ratio of 1:1600, incubating for 30 minutes at four degrees Celsius. The positive control is the Jurkat Cell Line. Again, pre-diluted forms shouldn’t be used for Flow Cytometry.
The CD45 market can help you research tumors and leukocytes. Visit Spring Bioscience to learn more.
